Hepatoprotective and nephroprotective activities of Solenostemon monostachyus P. Beauv (Lamiaceae) leaf extract
Abstract
Background: Solenostemon monostachyus P. Beauv (family Lamiaceae), a medicinal herb in West and Central Africa, is ethnomedically employed as an antidote for poison and for the treatment of different diseases and ailments.
Objective: To investigate the hepatoprotective and renoprotective effects of S. monostachyus leaf extract against paracetamol- induced liver and kidney injuries in rats.
Methodology: Hepato-renoprotective effects of S. monostachyus ethanol leaf extract was evaluated against paracetamol- induced liver and kidney injuries in rats. The liver protective property of the ethanol leaf extract (75-225 mg/kg) was investigated by the assessment of liver function parameters, liver antioxidant enzymes and histopathology, while the renoprotective property was evaluated by the assessment of some kidney function parameters, kidney antioxidant enzymes and histopathology. Silymarin (100mg/kg) was used as positive control.
Results : The leaf extract exerted significant (p<0.05 – 0.001) dose-dependent decreases in elevated levels of liver enzymes (ALT, AST and ALP), total cholesterol, direct and total bilirubin as well as increases in serum levels of total protein, albumin and antioxidant enzymes (SOD, CAT, GPx) and GSH. Histopathological study of the liver sections of extract and silymarin-treated rats revealed reductions in the pathological features compared to the paracetamol- treated animals. Leaf extract pre-treatment also resulted in significant (p<0.05) dose-dependent decreases in increased levels of serum creatinine and urea without affecting the electrolytes levels. Histopathology of the kidney sections of extract and silymarin- treated rats showed decreases in the pathological features compared to the control group. The chemical pathological results in both liver and kidney agreed with histopathological observations indicating pronouced hepatoprotective and renoprotective effect of the leaf extract of S. monostachyus.
Conclusion: The results of this study demonstrate that the leaf extract of S. monostachyus has the potentials to protect the liver and kidney against injury which may be due to its antioxidant activity of its constituents and this can be employed in the management of liver and kidney diseases.
Keywords: Solenostemon monostachyus, medicinal plant, hepatoprotective, renoprotective, antioxidant.
References
Adams R. P. (2001). Identification of Essential oils by Gas Chromatography Quadrupole Mass Spectrometry. Allured Publishing Corporation, Carol Stream, USA.
Adebayo JO, Krettli AU. (2011). Potential antimalarials from Nigerian plants: A review. J. Ethnopharmacol. 133: 289–302.
Ajibesin KK, Ekpo BA, Bala DN, Essien EE, Adesanya SA. (2008). Ethnobotanical survey of Akwa Ibom State of Nigeria. J. Ethnopharmacol. 115: 387–408.
Aldridge WN. (1981). Mechanism of toxicity: New concepts are required in toxicology. Trends Pharmacol. Sci. 2: 228–231.
Carpenter HM, Mudge GH. (1981). Acetaminophen nephrotoxicity: studies on renal acetylation and deacetylation. J. Pharmacol. Expl. Therap. 218: 161-167.
Curtis SJ, Moritz M, Snodgrass PJ. (1972). Serum enzymes derived from liver cell fractions. I. The response to carbon tetrachloride intoxication in rats. Gastroenterol. 62: 84 - 92.
Datte JY, Kpahe F, Offoumou AM. (2010). Acute toxicity and antioxidant activity of hydroethanolic extract of Solenostemon monostachyus P. Beauv. Leaves. J. Compl. Integr. Med. 7: 11-16.
Drotman RB, Lawhorn GT. (1978). Serum enzymes are indications of chemical induced liver damage. Drug Chem Toxicol. 1: 163 - 171.
Ekundayo EO, Ezeogu LI. (2006). Evaluation of antimicrobial activities of extracts of five plants used in traditional medicine in Nigeria. Intl J. Trop Med. 1: 93-96.
Ellman GL. (1959). Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82: 70-77.
Esterbauer H, Cheeseman KH. (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 186: 407–421.
Fernandez V, Videla IA. (1981). Effect of acute and chronic ethanol ingestion on the content of reduced glutathione on various tissues of the rat. Experientia. 37:392-394.
Fidele KZ, Andre KB, Yao DJ, Michel OA (2012). Action of hydroethanolic leaves extract of Solenostemon monostachyus (lamiaceae) on cardiovascular system of mammalians: blood pressure lowering effects Intl. J. Pharm. Biol. Sci. 2:310-320.
Gilani AH, Jabeen Q, Ghayur MN, Janbaz KH, Akhtar MS. (2005). Studies on the antihypertensive, antispasmodic, bronchodilator and hepatoprotective activities of the Carum copticum seed extract. J. Ethnopharmacol. 98: 127–135.
Husain K, Somani SM. (1997). Interaction of exercise and ethanol and hepatic and plasma antioxidant system in rat. Pathophysiol. 4:60-74.
Koffi N, Marie–Solange T, Emma AA, Noel ZG. (2009). Ethnobotanical study of plants used to reat arterial hypertension in traditional medicine, by abbey and Krobou population of Agboville (Cote d’ivoire).Eur. J. Sci. Res. 35:85-98.
Kumar PP, Kumaravel S, Lalitha C. (2010). Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr. J. Biochem. Res. 4: 191-195.
Lawrence RA, Burk RF. (1976). Glutathione peroxidase activity in selenium- deficient rat liver. Biochem. Biophys. Res. Comm. 71: 952-958.
Lin CC, Shieh DE, Yen MH. (1997). Hepatoprotective effect of the fractions of Ban-zhi-lian on experimental liver injuries in rats. J. Ethnopharmacol. 56: 193–200.
Lorke, D. (1983). A new approach to practical acute toxicity testing. Arch. Toxicol. 54:275-286.
Manokaran S, Jaswanth A, Sengottuvelu S, Nandhakumar J, Duraisamy R, Karthikeyan D, Mallegaswari R. (2008). Hepatoprotective activity of Aerva lanata Linn. Against paracetamol induced hepatotoxicity in rats. Res. J. Pharmaceut. Tech. 1: 398 – 400.
Marklund S, Marklund G. (1974). Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47: 469 - 474.
Martin P, Friedman LS. (1992). Assessment of liver function and diagnostic studies. In: Friedman LS, Keeffe EB. (Eds.), Hand Book of Liver Disease. Churchill Livingstone, Philadelphia, pp. 1–14.
Mugford CA, Tarlolf JB. (1997). The contribution of oxidation and deacetylation to acetaminophen nephrotoxicity in female Sprague-Dawley rats. Toxicol. Lett. 93: 15-18.
Mve-Mba CE, Menut C, Lamaty G, Zollo PHA, Tchoumbougnang. (1994). Aromatic plants of tropical central Africa. Part XIX. Volatile components from leaves of two lamiaceae from Cameroon: Leucas deflexa Hook. and Solenostemon monostachyus (P.Beauv.) Briq. Flav. Fragr. J. 9(6):315-317.
N’Guessan HA, Dago DCE, Mamyrbekova-Bekro JA, Békro YA. (2011). CCM d’extraits selectifs de 10 plantes utilisées dans le traitement traditionnel de l’hypertension arterielle en Côte d’Ivoire. Eur. J. Sci. Res. 66 : 575-585.
Nkosi CZ, Opoku AR, Terblanche SE. (2005). Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCl4-induced liver injury in low protein fed rats. Phytother. Res. 19: 341–345.
Okoko T, Ere D. (2012). Antioxidant activities of Solenostemon monostachyus leaf extract using in vitro methods. Sci. Res. Essays 7: 621-626.
Okokon JE, Davis K, Nwidu LL. (2016). Analgesic and antiinflammatory activities of Solenostemon monostachyus. Avic. J. Phytomed. 6:284-394.
Okokon JE, Davis K, Azare B. (2016). Antimalarial and antipyretic activities of Solenostemon monostachyus. Pharm. Biol. 54:648 - 653.
Reitman S, Frankel S. (1957). Determination of Glutamate-Pyruvate transaminase (ALT) and Aspartate Aminotransfrase (AST). J. Clin. Pathol. 28: 56.
Setzer, W.N., Stokes, S. L., Penton, A. F., Takaku, S., Haber, W. A., Hansell, E., Caffrey, C. R., McKerrow, J. H. (2007). Cruzain Inhibitory activity of leaf essential oils of neotropical lauraceae and essential oil components. Nat. Prod. Comm. 2: 1203 – 1210.
Sinha AK. (1972). Colorimetric assay of catalase. Analy. Biochem. 47: 389-394.
Sofowora, A. 1993. Medicinal Plants and Traditional Medicine in Africa. 2nd edn, Spectrum Book Ltd, Ibadan, Nigeria.
Toshio M, Peter R, Conrad HE. (1980). Structures of six Coleons (Diterpenoids) From Solenostemon monostachyus (P. Beauv.) Briq. (Labiatae). Helvetica Chimica Acta- 63: 95-101.
Trease GE, Evans WC. (1989). Pharmacognosy, 13th ed. Bailliere Tindal, London.
Wilbur KM, Bernhein F, Shapiro OW. (1949). Determination of lipid peroxidation. Arch. Biochem. 24:305-10.
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