Antinociceptive and anti-inflammatory activities of root extract of Zea mays
Abstract
Background: Zea mays is used in Ibibio traditional medicine in Nigeria for the treatment of various ailments such as pains, malaria and hemorrhoids.
Objective: To evaluate the anti-inflammatory and analgesic activities of Zea mays ethanol root root extract in mice.
Methodology: The crude ethanolic extract (45 – 135 mg/kg) of Zea mays root was investigated for anti-inflammatory and analgesic activities in mice using various experimental models; acetic acid and thermal- induced pains and carrageenan, egg albumin and xylene – induced oedema. The activity of the extract was compared to that of standard drug, acetyl salicylic acid (100 mg/kg).
Results: The extract caused a significant (p<0.05 – 0.001) dose-dependent reduction of inflammation and pains induced by different phlogistic agents used. These effects were comparable to that of the standard drugs, acetyl salicylic acid (100 mg/kg) used in some models.
Conclusion: The anti-inflammatory and analgesic effects of this plant may in part be mediated through the chemical constituents of the plant and the results of the analgesic action suggest central and peripheral mechanisms. The findings of this work confirm the ethno medical use of this plant to treat inflammatory conditions.
Key words: Zea mays, antiinflammatory, analgesic
References
Abo KA, Fred-Jaiyesimi AA, and Jaiyesimi AEA (2008). Ethnobotanical studies of medicinal plants used in the management of diabetes mellitus in South Western Nigeria. J. Ethnopharmacol. 115: 67-71.
Akah PA and Njike HA (1990). Some pharmacological effects of rhizome aqueous extract of Anchomanes diformis. Fitoterapia 60: 368-370.
Akah PA and Nwanbie A (1994). Evaluation of Nigerian traditional medicines plants used for rheumatic (inflammatory) disorder. J. Ethnopharmacol. 42: 179 – 182.
Amico-Roxas M, Caruso A, Trombadore S, Scifo R and Scapagnime, U. (1984). Gangliosides antinociceptive effects in rodents. Arch. Intl. Pharmacodynam. Therapeut. 272: 103-117.
Balasubramanian K and Padma PR. (2013). Anticancer activity of Zea mays leaf extracts on oxidative stress-induced Hep2 Cells. J Acupunct. Meridian Stud. 6:149-158.
Balasubramanian K, Padma PR. (2012). Screening of antioxidant properties of Zea mays Leaves at different time periods of growth J. Pharm. Res. 5:4034-4037.
Balasubramanian K, Vidhya A, Thiruselvi M, Sudhadevi M and Padma PR. (2014). Zea mays leaf extracts exhibits anticancer property and enhance the chemotherapeutic action of etoposide in cancer cells. Indo Amer. J. Pharm Res. 4: 1530 - 1539.
Balasubramanian K, Jincy PA and Padma PR (2015). Influence of methanolic extract of Zea mays leaves against CCL4 and H2O2 induced oxidative stress in Drosophila melanogaster. Indo Amer. J. Pharm Res. 5: 566-577.
Bentley GA, Newton SH and Starr J. (1983). Studies on the antinociceptive action of agonist drugs and their interaction with opoid mechanisms. Br. J. Pharm. 79: 125 - 134.
Besra SE, Sharma RM and Gomes A. (1996). Antiinflammatory effect of petroleum ether extract of leaves of Litchi Chinensis. Caertn (sapindaceae). J. Ethnopharmacol. 54:1-6.
Carlo Di G, Mascolo N, Izzo AA and Capasso F (1999). Flavonoids, old and new aspects of a class of natural therapeutic drugs. Life Sci. 65:337–353.
Correa CR, Kyle DJ, Chakravarty S, Calixto JB. (1996). Antinociceptive profile or the pseudopeptide β2 bradykinin receptors antagonist NPC 18688 in mice. Br J. Pharmacol. 117:552-556.
Deraedt R, Jougney S and Falhout M. (1980). Release of Prostaglandin E and F in an algogenic reaction and its inhibition. Eur J Pharm. 51:17-24.
Dong J, Cai L, Zhu X, Huang X, Yin T, Fang H and Ding Z (2014). Antioxidant activities and phenolic compounds of cornhusk, corncob and Stigma Maydis. J. Braz. Chem. Soc. 25: 1956-1964.
Ekpendu TO, Akah PA, Adesomoju AA and Okogun JI. (1994). Antinflammatory and antimicrobial activities of Mitracarpus scaber extracts. Intl. J. Pharmacol. 32:191-195.
Foster S, Duke JA. (1990). Field Guide 10 Medical Plants: Eastern and Central North America. Houghton MifAin, Boston.
Franzotti EM, Santos CVF, Rodrigues HMSL, Mourao RHV, Andrade MR and Antoniolli AR. (2002). Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. J Ethnopharmacol 72: 273-278.
Gill LS. (1992). Ethnomedical Uses of Plants in Nigeria. Uniben Press, Benin, Nigeria, p. 249.
Hess SM and Milonig RC. (1972). Inflammation In: Lepow LH, Ward PS. (Eds). Inflammation, Mechanism and control. Academic Press, New-York, USA. pp.1-2.
Huss U, Ringbom T, Perera P, Bohlin L and Vasange M. (2002). Screening of ubiquitous plant constituents for COX-2 inhibition with a scintillation proximity based assay. J. Nat. Prod. 65: 1517-1521.
Krogh R, Kroth R, Berti C, Madereira AO, Souza MM, Cechinel-Filho V, Delle-Monache F and Yunes RA. (1999). Isolation and identification of compounds with antinociceptive action from Ipomoea pes-caprae. Pharmazie 54: 464 – 466.
Liang YC, Huang YT, Tsau SH, Lin-Shiau SY, Chen CF and Lin JK. (1999). Suppression of inducible cyclo-oxygenase and inducible nitric acid synthase by apigenia and related flavonoid in mouse macrophages. Carcinogenesis. 20: 1945-52.
Lin LL, Lin AY and Knopt JL. (1992). Cytosolic phospholipase A2 is coupled to hormonally regulated release of arachidonic acid. Proc. Nat. Acad. Sci., U.S.A. 89:6147-6157.
Liu J (1995). Pharmacology of oleanolic acid and ursolic acid. J. Ethnopharmacol. 49: 57- 68.
Lorke D (1983). A new approach to practical acute toxicity testing. Arch. Toxicol. 54:275-286.
Maia JL, Lima-Junior RC, David JP, David JM, Santos FA and Rao VS (2006). Oleanolic acid, a pentacyclic triterpene attenuates the mustard oil induced colonic nociception in mice. Biol. Pharmaceut. Bull. 29: 82–85.
Middleton E Jr, Kandaswami C and Theoharides TC (2000). The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacol. Rev. 52: 673–751.
Nwafor PA, Jacks TW and Ekanem AU. (2007). Analgesic and anti-inflammatory effects of methanolic extract of Pausinystalia mecroceras stem bark in rodents. J. Pharmacol. 3:86-90.
Nwafor PA, Nwajiobi N, Uko IE and Obot JS (2010). Analgesic and anti-inflammatory activities of an ethanol extract of Smilax krausiana leaf in mice. Afr. J. Biomed. Res. 13: 141 -148.
Okokon JE and Nwafor PA (2010). Antiinflammatory, analgesic and antipyretic activities of ethanolic root extract of Croton zambesicus. Pak. J. Pharmaceut. Sci. 23: 383 - 390.
Oriowo MA. (1982). Anti-inflammatory activity of piperonyl,-4-acrylic isobutyl amide, an extractive from Zanthoxylum zanthoxyloids. Planta Medica 44: 54 – 56.
Osagie AU and Eka OU (1998). Nutritional Quality of Plant Foods. Post Harvest Research Unit, University of Benin, Benin, Nigeria, pp 34-41.
Owoyele BV, Negedu MN, Olaniran SO, Onasanwo SA, Oguntoye SO, Sanya JO, Oyeleke SA, Ibidapo AJ and Soladoyel AO (2010). Analgesic and anti-inflammatory effects of aqueous extract of Zea mays husk in male wistar rats J. Med. Food 13:343-347.
Park S, Takano Y, Matsuura H and Yoshihara T. (2004). Antifungal compounds from the root and root exudate of Zea mays. Biosci. Biotech. Biochem. 68:1366-1368.
Rajendran NN, Thirugnanasambandam P, Viswanathan S, Parvathavarthini S and Ramaswamy S. (2000). Antinociceptive pattern of flavone and its mechanism as tested by formalin assay. Ind. J. Expl Biol. 38: 182–185.
Ringbom T, Segura L, Noreen Y, Perera P and Bohlin L (1998). Ursolic acid from Plantago major, a selective inhibitor of cyclooxygenase-2 catalysed prostaglandin biosynthesis. J. Natl Prod. 61: 1212–1215.
Robak J, Shridi F, Wolbis M and Krolikowska M. (1998). Screening of the influence of flavonoids on lipoxygenase and cyclooxygenase activity, as well as on nonenzymic lipid oxidation. Pol. J. Pharmacol. Pharm. 40: 451–458.
Santos AR, Cechinel Filho V, Niero R, Viana AM, Moreno FN, Campos MM, Yunes RA and Calixto JB (1994). Analgesic effects of callus culture from selected species of Phyllanthus. J. Pharm Pharmacol. 46: 755 – 759.
Simmonds NW (1979). Evolution of Crop Plants. Longman. London. pp. 128-129.
Sofowora A. Medicinal Plants and Traditional Medicine in Africa. 2nd edn, Spectrum Book Ltd, Ibadan, Nigeria. 1993.
Suh HW, Song DK, Son KH, Wie MB, Lee KH, Jung KY, Do JC and Kim YH (1996). Antinociceptive mechanisms of dipsacus saponin C administered intracerebroventricularly in the mouse. Gen. Pharmacol. 27: 1167–1172.
Suh N, Honda T, Finaly HJ, Barchowsky A, Williams C and Benoit NE (1998). Novel triterpenoids suppress inducible nitric oxide synthase (iNOS) and inducible cyclooxygenase (COX-2) in mouse macrophages. Cancer Res. 58:717-723.
Trease GE. and Evans WC. Pharmacognosy, 13th ed. Bailliere Tindal, London. 1989.
Tapondjou LA, Lontsi D, Sondengam BL, Choi J, Lee KT, Jung HJ and Park HJ (2003). In vivo antinociceptive and antiinflammatory effect of the two triterpenes, ursolic acid and 23-hydroxyursolic acid, from Cussonia bancoensis. Arch. Pharm. Res. 26: 143–146.
Tjolsen A, Berge OG, Hunskaar S, Rosland JH and Hole K (1992). The formalin test: An evaluation of the method. Pain 51:5-17.
Turner RA. (1995). Screening methods in Pharmacology. Vol 1. Academic Press. New York. Pp. 85- 106.
Vane T and Booting R. (1987). Inflammation and mechanism of action of anti- inflammatory drugs. FASSEB J. 1:89-96.
Vaz ZR, Cechinel V, Yunes RA and Calixto JB. 1996. Antinociceptive action of 2-(4-bromobenzoyl)-3-methyl-4-6-dimethoxy bezofuran, a novel xanthoxyline derivative of chemical and thermal models of nociception in mice. J. Pharm. Expl Therapeut. 278: 304 - 312.
Winter CA, Risley EA, Nuss GW (1962). Carrageenan-induced oedema in hind paw of the rats as an assay of anti-inflammatory drugs. Proceed. Soc. Expl Biol. Med. 111:544 -547.
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