LEVELS OF MALONDIALDEHYDE, GLUTATHIONE AND ASCORBIC ACID IN IDIOPATHIC THROMBOCYTOPAENIC PURPURA
Abstract
Background: Idiopathic thrombocytopaenic purpura (ITP) is an autoimmune disorder,
which causes an acute or chronic thrombocytopenia, and may result in potentially lifethreatening
haemorrhage. Oxidative damage may be involved in the pathogenesis of
autoimmune diseases. Antibodies to bind to membrane lipids and platelet destruction
may play a role on lipid peroxidation in ITP.
Objectives: To investigate the posible role of lipid peroxidation and antioxidants in
patients with ITP.
Design: The levels of plasma and erythrocyte malondialdehyde (MDA), erythrocyte
glutathione and ascorbic acid were analysed in patients with ITP.
Methods. The MDA levels were performed according to the method of Bidlack WR.
Plasma MDA, erythrocyte glutathione and ascorbic acid levels were carried out
according to the methods of Ohkawa H, Beutler E and Bauer JD, respectively.
Results: The erythrocyte and plasma MDA levels in patients with ITP were found to
be 9.52±4.65, 3.03±1.44 (p<0.001) and in control group were found to be 2.49±0.57,
1.03±0.28 nmol/ml (p<0.001), respectively. Erythrocyte glutathione was found to be
3.71±0.82, 6.26±0.66 µmol/gr Hb (p<0.001). Ascorbic acid levels of these groups were
1.09±0.25, 1.70±0.33 mg/dl (p<0.001).
Conclusion: The oxidative damage is involved in the pathogenesis of ITP. In patients
with ITP, the platelet destruction and bleeding may play significant role on elevation
of lipid peroxidation and reduction of antioxidant capacity. Further studies on oxidant
and antioxidant status of ITP are also needed to confirm these results.
which causes an acute or chronic thrombocytopenia, and may result in potentially lifethreatening
haemorrhage. Oxidative damage may be involved in the pathogenesis of
autoimmune diseases. Antibodies to bind to membrane lipids and platelet destruction
may play a role on lipid peroxidation in ITP.
Objectives: To investigate the posible role of lipid peroxidation and antioxidants in
patients with ITP.
Design: The levels of plasma and erythrocyte malondialdehyde (MDA), erythrocyte
glutathione and ascorbic acid were analysed in patients with ITP.
Methods. The MDA levels were performed according to the method of Bidlack WR.
Plasma MDA, erythrocyte glutathione and ascorbic acid levels were carried out
according to the methods of Ohkawa H, Beutler E and Bauer JD, respectively.
Results: The erythrocyte and plasma MDA levels in patients with ITP were found to
be 9.52±4.65, 3.03±1.44 (p<0.001) and in control group were found to be 2.49±0.57,
1.03±0.28 nmol/ml (p<0.001), respectively. Erythrocyte glutathione was found to be
3.71±0.82, 6.26±0.66 µmol/gr Hb (p<0.001). Ascorbic acid levels of these groups were
1.09±0.25, 1.70±0.33 mg/dl (p<0.001).
Conclusion: The oxidative damage is involved in the pathogenesis of ITP. In patients
with ITP, the platelet destruction and bleeding may play significant role on elevation
of lipid peroxidation and reduction of antioxidant capacity. Further studies on oxidant
and antioxidant status of ITP are also needed to confirm these results.
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