Antiplasmodial, Cytotoxic and Acute Toxicity Activities of Vernonia lasiopus O. Hoffman

Abstract

Background: Malaria continues to cause heavy morbidity and mortality and it is the fifth leading cause of death globally. The disease causes over one million deaths annually and affects many more, particularly due to increasing multi-drug resistant strains of Plasmodium falciparum. Sustained investigations in both curative and prophylactic interventions have supported the ethno-pharmacological approach to identify novel compounds as a major channel towards achieving a solution. Vernonia lasiopus has been used in traditional medicine for their antimalarial, antiviral and analgesic properties.

Objective: To investigate the antiplasmodial activity and toxicity profile Vernonia lasiopus extracts.

Methodology: Extraction of aerial parts and roots was done using dichloromethane:chloroform (1:1) and the resulting crude extracts each fractionated into six fractions by vacuum liquid chromatography using solvents of different polarities. The crude extract and fractions were investigated for antiplasmodial activity using the chloroquine (CQ) sensitive D6 and chloroquine (CQ) resistant W2 laboratory adapted Plasmodium falciparum strains. Cytotoxicity was evaluated on Vero 199 cells at starting concentrations of 100µg/ml, whereas acute toxicity (LD₅₀) determined on healthy female Swiss mice (20±2 gm.). Selectivity index was used as an indicator of antiplasmodial viability.

Results: The fractions of V. lasiopus roots showed higher activity combined than individually. The crude V. lasiopus root extract had an IC₅₀ 13.1 µg/ml and selectivity index >7.63. Fraction 1 of the crude root extract (VLR1) was the most viable fraction with an IC₅₀ of 16.8 µg/ml and S.I >5.95. Both had CC₅₀>100 µg/ml and LD₅₀ >5000mg/kg.

Conclusion: extracts of V. lasiopus aerial parts and roots were found to exhibit notable viable antiplasmodial effects, and had minimal acute toxicity in mice.

Key words: Plasmodium falciparum, Vernonia lasiopus, antiplasmodial activity, toxicity, selectivity index.